Pressure-sterilized nutrient broth pre-loaded into canning jars with self-healing injection-port lids, plus sterile syringes for drawing the finished liquid culture. Everything needed to take a single inoculation source (one spore syringe or one LC syringe) and turn it into many syringes of live, ready-to-use liquid culture.

Plan on 2–3 weeks from inoculation to a fully matured jar of liquid culture. Inject the broth with your starting source, agitate gently every few days to keep mycelium suspended in the broth, and incubate at approximately 76°F in a clean environment. When the broth has visible cloudy mycelial growth and no off-colors, it's ready to draw into syringes.

Sealed, refrigerated liquid culture in a syringe stays viable for many months — in practice indefinite, as long as the sterile seal holds. The mycelium becomes dormant in cold storage and resumes activity when warmed. Mature LC in the original broth jar is best used within 6–12 months refrigerated; broth nutrients deplete over time.

Not for the kit itself — the included jars work without one. A stir plate accelerates the process by keeping mycelium suspended in the broth (faster nutrient access, faster colonization) and is the standard upgrade for cultivators scaling LC multiplication. Pair with GL45 ported caps when converting to a stir-plate workflow on laboratory glass bottles.